壳聚糖-纳米羟基磷灰石复合材料修复兔胫骨缺损的成骨效果

摘要

目的 探讨壳聚糖-纳米羟基磷灰石复合材料(CTS-nHA)植入动物体内修复骨缺损时,观察成骨效果和材料吸收速度.方法 新西兰大白兔18只,随机分为3组,实验组(A组):复合材料;实验对照组(B组):纳米羟基磷灰石(N-HA);空白对照组(C组):不植入任何材料,于兔双侧后肢胫骨制造约直径0.5 cm大小的骨缺损,分别植入相应的材料或不植入任何材料,于术后2、4、6、10周取骨缺损标本,进行大体和组织学观察.结果 在各时间段CTS-nHA组新的骨基质生成量均明显高于n-HA组和空白组,其中n-HA组又高于空白组,只有在2周时n-HA组和空白组相似.同时复合材料在体内吸收速度比n-HA组快,复合材料的吸收与新骨生成速度协调性似乎欠佳.结论 复合材料植入的缺损区具有较强的成骨活性,但在体内吸收速度比较快.%Aim To explore the effect of bone formation of chitosan-HA composite materials and its speed of absorption during rabbits Tibial bone defect repair. Method 18 adult New Zealand rabbits were randomly divided into 3 groups:Chitosan/HA group,HA group, and post-operative group. After 2,4,6 and 10 weeks of post-operation,rabbits were operated with lcm osteomoty at two tibial bone. The materials were embeded into the defect position respectively. After 2,4,6 and 10 weeks,the bone defect positions were operated and were observed pathogically. Results There was more abundant new bonematrix migrating into Chitosan/HA group than into other two groups at each week we have observed( P <0.01 ). At the same time, more abundant new bonematrix migrating into the HA group than the control group( P <0. 01 ). The speed at which material of Chitosan/HA group absorded is faster than that of HA group, and the speed at which material of Chitosan/HA group absorbed was also faster than the speed of new bonematrix migrating. Conclusion Chitosan/HA composite materials probably have good osteoinductive activity,and the absorption speed is faster.