Objective To study the effects of hydroxyapatite/ chitosan/ poly lactic composite in repair of bone de-fect. Methods Eighteen 3-month-old New Zealand white rabbits were randomly divided into experimental group and control group. Prepared 15 mm complete bone defect model at the bilateral radius, the experimental groups were implanted with HA/CS/PLLA composite and control groups were implanted nothing into the bone defect site. The bone defects of the animals were observed at the end of 4th week, 8th week and 12th week by anatomy, X-ray and histology methods, respectively. Results All the 18 rabbits entered the final analysis and no apparent reject reaction was found. At the end of the 12th week after operation the experimental group bone defects were repaired completely. However, the control group bone defects were not partially repaired. At the end of the 4th week, X-ray showed that osteotylus was seen in the defect area in the experimental group, and implants were mixed with the bone tissue of defects, and cortical continuity was better at the 12th week, the control group showed bone defect had not been partially repaired. Histological examination showed that lots of osteoblasts were seen in implants in the experimental group at the 4 th week, and began to connected to each other. At the 12th week, more woven bone-1 ike tissues were visible and trabecular-like structure was formed. Conclusion HA/ CS/ PLLA composite has good osteogenic potential and may be used to repair bone defect.%目的了解羟基磷灰石/壳聚糖/聚乳酸(HA/CS/PL-LA)骨修复材料的生物相容性,初步探讨其作为骨替代材料的可行性。方法3月龄新西兰大白兔18只,每只兔子的左右前肢按随机法分为实验组和对照组。在双侧桡骨制备长约15 mm的完全性骨缺损,实验组植入HA/CS/PLLA骨修复材料,对照组为空白对照,骨缺损部位不植入任何材料。术后4、8、12周取标本,采用大体标本、X线和组织学方法检测缺损部位的成骨情况。结果18只兔子均纳入分析,复合材料植入区未见明显排斥反应。术后12周时实验组骨缺损区修复完整；对照组骨仍有缺损未修复。 X线片结果显示：4周时实验组桡骨缺损区可见均匀分布的云雾状骨生成影像,随时间的延长,至12周时,植入体已与缺损处断端骨性愈合,皮质骨连续性较好；对照组12周时显示骨缺损区部分修复。组织学结果表明,4周时实验材料内部可见大量骨形成细胞集中,并开始相互连接成片,到12周时,材料内骨小梁成熟,新生骨小梁相互连接成板状。结论HA/CS/PL-LA复合材料具有良好的生物相容性和成骨能力。
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