替普瑞酮及雷贝拉唑对急性胃黏膜损伤大鼠 TFF3表达的研究

摘要

Objective To explore the expression of TFF3 and the protective role of Teprenone and Rabeprazole in rats with acute gastric mucosal lesion.Method Forty wistar male rats were randomly assigned into normal control group, model group, teprenone group, rabeprazole group and teprenone/rabeprazole group, with 8 rats in each group.Acute gastric mucosal lesion model was induced by ab-solute ethylalcohol.Gastric tissue pathological changes were performed by hematoxylineosin staining,and gastric ulcer index was evalu-ated.Expression of TFF3 in gastric mucosa was detected by immune histochemical method.Results (1) UI of drug treatment groups was significantly lower than that of model group(P<0.01);UI of combination group was lower than that of teprenone group(P<0. 05);UI of combination group was lower than that of rabeprazole group(P<0.01).(2) Hematoxylineosin staining results were as fol-lows:Compared with control group, epithelial cells were deficient on gastric mucosa surface in model group, with a large number of in-flammatory cells infiltration as well as muscularis and serosa destroyed; By comparison with model group, in rabeprazole group, te-prenone group and rabeprazole/teprenone group deficiency of epithelial cells of gastric mucosa surface was less, mucous glands reap-peared, and serous membrane structure was relatively complete with less inflammatory cells and exudate.(3)Immunohistochemical re-sults were as follows:Expression of TFF3 positive cells in model group and drug intervention group was significantly increased, which was significantly higher than that in normal control group(P<0.05).Conclusion Rabeprazole and teprenone may protect gastric mu-cosa tissues by raising TFF3 expression,promote the healing of gastric mucosa and alleviate the acute gastric mucosa injury in rats.%目的：研究替普瑞酮及雷贝拉唑对无水乙醇诱发大鼠急性胃黏膜病变的保护性作用及三叶草因子3（ TFF3）的表达。方法将大鼠按体重随机分为5组，分为正常对照组、模型组、替普瑞酮治疗组、雷贝拉唑治疗组、替普瑞酮联合雷贝拉唑治疗组（联合治疗组），每组8只。用无水乙醇制备大鼠急性胃黏膜病变模型，苏木精—伊红染色观察各组大鼠胃黏膜组织病理学改变，免疫组织化学法测定各组大鼠胃黏膜TFF3的表达情况。结果与模型组比较，各药物治疗组胃黏膜溃疡指数明显减少，有显著性差异（P＜0．01）；与替普瑞酮治疗组比较，联合治疗组胃黏膜溃疡指数明显减少，有统计学意义（P＜0．05）；与雷贝拉唑治疗组比较，联合治疗组胃黏膜溃疡指数明显减少，有显著性差异（ P＜0．01）。 HE染色结果，与正常对照组比较，模型组大鼠的胃黏膜表面上皮细胞缺失，伴随大量炎症细胞浸润，肌层和浆膜被破坏；与模型组比较，雷贝拉唑、替普瑞酮治疗组及联合治疗组的大鼠胃黏膜表层上皮细胞缺失减少，黏膜腺体重现，浆膜结构较完整，且炎症细胞较少。免疫组织化学结果，与正常对照组比较，模型组及各药物治疗组胃黏膜TFF3的表达显著增多（ P＜0．05）。与雷贝拉唑组比较，联合治疗组大鼠胃溃疡周边TFF3的阳性细胞密度明显增加（P＜0．05）。结论替普瑞酮与雷贝拉唑可能通过上调 TFF3的表达改善无水乙醇所致大鼠胃黏膜组织损伤，促进胃黏膜的愈合，对大鼠急性胃黏膜病变起到保护作用。