复方二黄颗粒对肾衰大鼠肾脏纤维化的抑制作用

摘要

目的：观察复方二黄颗粒对慢性肾衰竭大鼠的肾组织病理及 TGF-β1（转化生长因子-β1）、FN（纤维连接蛋白）、α-SMA （α-平滑肌肌动蛋白）表达的影响。方法成年雄性 SD 大鼠30只，随机分为假手术组、模型对照组、治疗组共3组；5／6肾切除喂养4周后，各组大鼠分别给予药物及等量生理盐水灌胃共8周后，处死所有大鼠，留取肾组织。观察各组大鼠的肾脏病理变化，并用免疫组化法观察肾组织 TGF-β1、FN、α-SMA 的表达，并进行统计学分析。结果复方二黄颗粒能改善肾衰大鼠的肾小球硬化、间质纤维化；并能抑制肾衰大鼠肾组织中 TGF-β1、FN、α-SMA 表达的上调，各组间比较有统计学意义（P ＜0．05）。结论复方二黄颗粒能改善慢性肾衰竭大鼠的肾脏纤维化。%Objective To observe the effects of Erhuang compound granule on protecting and curing chronic renal failure (CRF)due to 5 /6 nephrectomy (NPX).Methods Thirty rats with 5 /6 nephrectomy were assigned into 3 groups:nonoperation group,excision mod-el group and Erhuang compound granule group.Then,each group was fed with different medicines for 8 weeks before the rats were killed and the kidneys were used for pathological testing.Results Erhuang compound granule could significantly down -regulate protein ex-pressions of TGF-β1 ,FN and α-SMA in kidney of CRF rats,which could improve the condition of the CRF rats.Conclusion Erhuang compound granule is protective for chronic renal failure in kidney subtotal excision rats.