甘草黄酮对脓毒症急性肺损伤大鼠的保护作用

摘要

目的：探讨甘草黄酮对脓毒症急性肺损伤大鼠的保护作用。方法制作脓毒症急性肺损伤模型大鼠60只。将大鼠随机分为甘草黄酮组（n ＝30）和对照组（n ＝30），甘草黄酮组给予10 mg·kg －1甘草黄酮治疗，对照组给予等剂量生理盐水。主要观察指标为两组大鼠48 h 死亡率、氧分压（PaO2）、二氧化碳分压（PaCO2）、pH、白细胞介素-1（IL-1）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）。结果两组大鼠术前及术后1 h、PaO2、PaCO2、pH、IL-1、IL-6和 TNF-α等方面差异无统计学意义（P ＞0．05）。但与对照组比较，甘草黄酮组大鼠术后24 h 甘草黄酮组大鼠 PaO2显著增高（71．3±11．2 vs 55．2±9．4 mmHg， P ＝0．000）；PaCO2显著降低（39．6±9．4 vs 54．5±12．6 mmHg，P ＝0．000）；pH 显著增高（7．31±0．17 vs 7．11±0．08， P ＝0．018）；IL-1显著降低（0．34±0．13 vs 0．62±0．21μg·L －1，P ＝0．000）；IL-6显著降低（180±56 vs 390±121 ng·L －1，P ＝0．000）；TNF-α显著降低（2．23±1．09 vs 5．32±1．43μg·L －1，P ＝0．000）；术后48 h 死亡率显著降低（13．33％ vs 43．33％， P ＝0．010）。结论甘草黄酮显著改善了脓毒症急性肺损伤大鼠肺通气功能，降低了大鼠全身炎性反应和术后48 h 死亡率。%Objective To investigate the protective effect of licoflavone in rats with sepsis and acute lung injury.Methods Sixty model rats of acute lung injury induced by sepsis were made.All rats were randomized into licoflavone group (n =30)or control group(n =30).Licoflavone (10 mg·kg -1 )was given to the licoflavone group while 10 mg·kg -1 saline was given to the control group.The pri-mary outcomes were 48-hour postoperative mortality,partial pressure of oxygen,partial pressure of carbon dioxide,pH,IL-1,IL-6 and TNF-α.Results There were no significant differences between two groups in preoperative and 1-hour postoperative partial pressure of oxygen,partial pressure of carbon dioxide,pH,IL-1,IL-6 and TNF-α(P >0.05).However,when compared to the control group,rats in licoflavone group got a significantly higher level of partial pressure of oxygen (71.3 ±11.2 vs 55.2 ±9.4 mmHg,P =0.000),a signifi-cantly lower level of partial pressure of carbon dioxide (39.6 ±9.4 vs 54.5 ±12.6 mmHg,P =0.000),a significantly higher level of pH (7.31 ±0.17 vs 7.11 ±0.08,P =0.000),a significantly lower level of IL-1 (0.34 ±0.13 vs 0.62 ±0.21 μg·L -1 ,P =0.000), a significantly lower level of IL-6 (180 ±56 vs 390 ±121 ng·L -1 ,P =0.000),a significantly lower level of TNF-α(2.23 ±1.09 vs 5.32 ±1.43 μg·L -1 ,P =0.000),and a significantly lower postoperative mortality (13.33% vs 43.33%,P =0.010).Conclusions Licoflavone improved the respiratory function of rats with acute lung injury induced by sepsis,and decreased the systemic inflammato-ry response and 48-hour postoperative mortality.