同种种苗异地栽培丹参质量比较研究

摘要

Objective To analyze and evluate the quality of Salvia miltiorrhiza cultivated in different areas.Methods Based on the method in the Chinese Pharmacopoeia 2015 Edition using high performance liquid chromatography (HPLC) to determine the contents of salvianolic acid B and tanshinone (tanshinone ⅡA,cryptotanshinone,tanshinone I) from six parts of Salvia miltiorrhiza planted in two different places,including fibrous roots,stems,leaves,flowers and receptacle.Results The contents of the two kinds of components of salvia miltiorrhiza cultivated in Hanshan are slightly higher than those cultivated in Taihe.The distribution content order of tanshinones from high to low is root,fibrous roots,receptacle,stem,leaf and flowers and the distribution content order of salvianolic acid B from high to low is root,fibrous roots,flowers,receptacle,stem and leaf.There is an obvious difference in contents from different parts of danshen and the contents of root and fibrous roots are higher than the standards of pharmacopoeia.Conclusions The contents of lipid-soluble components cultivated in different areas have significant differences.Salvia miltiorrhiza cultivated in Hanshan is superior to that in Taihe.There is no significant difference in contents of water-soluble components.The experiment can provide the reference in accordance with the expansion of planting of seedlings from areas with good quality.%目的 对同种丹参种苗在不同地区栽培的丹参质量进行分析评价.方法 根据2015年版药典方法,采用高效液相色谱法(HPLC),对同种种苗异地栽培丹参的根、须根及地上部分茎、叶、花、花托6个部位的丹酚酸B、丹参酮类(丹参酮ⅡA、隐丹参酮、丹参酮I)进行含量测定.结果 同种种苗的含山栽培丹参中两类成分含量均略高于太和栽培丹参,丹参酮类在不同部位分布的含量为根>须根>花托>茎>叶>花,丹酚酸B则为根>须根>花>茎>花托>叶,丹参不同部位含量有较大差异,其中根和须根的含量均高于药典标准.结论 异地栽培的丹参脂溶性成分差异有统计学意义,含山产栽培丹参优于太和产;异地栽培的丹参水溶性成分丹参含量无差异;该实验为指导优质产区种苗的扩大种植提供参考依据.