人尿中形态砷的高效液相色谱-氢化物发生-原子荧光光谱法联用分析

摘要

High performance liquid chromatography-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS) was used for analyzing four arsenic species [As( Ⅲ) , As( V ) , MMA and DMA] in human urine. The separation conditions for each arsenic species were investigated. The method is simple, sensitive, precise, as well as timesaving, and can be used for quantitative analysis of arsenic species in urine samples.%采用高效液相色谱-氢化物发生-原子荧光光谱法(HPLC-HG-AFS)测定了人尿中4种砷化物[As(Ⅲ)、As(V)、MMA和DMA],考察了各种砷化物分离的条件.该方法具有操作简便、灵敏度高、准确度高、节省时间等特点,可以对尿液样品中各形态砷进行准确的定量分析.