高效液相色谱法测定注射用法罗培南钠中还原型谷胱甘肽的含量

摘要

目的：建立注射用法罗培南钠中还原型谷胱甘肽的含量测定方法。方法：采用高效液相色谱法，C18色谱柱，以辛烷磺酸钠磷酸盐缓冲溶液-甲醇（90∶10）为流动相；DAD 检测器，210nm 波长处测定。结果：还原型谷胱甘肽与其他成分均完全分离，在考察的浓度范围内线性关系良好（r ＝0．9997），回收率符合规定。结论：该方法专属性好，准确度高，可作为注射用法罗培南钠中还原型谷胱甘肽的含量测定方法。%Objective :An accurate method to determine the content of reduced glutathione in faropenem sodium for injection .Methods :A C18 column was used for the separation .The mobile phase was octane-sulfonic acid sodium - phosphate buffered solution and methanol (90 ∶ 10) .The wavelength of DAD was 210nm .Results :The linearity was good in each range of concentration (r = 0.9997) .The recoveries were all accorded with prescribe .Conclusion :The method is accurate and specific for determination of reduced glutathione in faropenem sodium for injection .