Determination of Reduced Glutathione by High Performance Liquid Chromatography in Patients with Renal Stones and Diabetes Mellitus Type 2.

摘要

ABSTRACT High- Performance Liquid Chromatography Technique of Fluorescence Detector ( HPLC-FLD) was used for the detection of sulfur glutathione disulfide (GSSG) based on a derivatization glutathione with orthophthalaldehyde (OPA) at pH 12. This technique is characterized by high sensitivity, high specificity , and high selectivity for many of the compounds and in particular the glutathione in serum samples of patients with renal stones. Standard glutathione (20.