猪圆环病毒2型201105ZJ分离株的遗传变异分析

摘要

Objective] This study aimed to investigate the genetic variation of porcine circovirus type 2 (PCV2) in China. [Method] The strain was isolated from infected samples by cel passage and preliminarily identified by PCR and IFA. Ful-length genome of the isolated strain was obtained by specific amplification for homology and phylogenetic analysis. [Result] A PCV2 strain was successful y isolated and named 201105ZJ, which could proliferate in PK15 cel lines. Specific fragments could be amplified by specific PCR assay. According to results of IFA assay, specif-ic immunofluorescence was observed; the TCID50 was low (102.67); the ful-length genome sequence of the isolated strain was 1 768 bp, sharing 94.1%-96.8% ho-mology with 13 reference strains; to be specific, the isolated strain exhibited the highest homology of 96.8% with AF055392PCV2a; the isolated strain 201105ZJ and reference strain AF055392 belonged to genotype PCV2a, exhibiting a distant genetic relationship with genotype PCV2c. [Conclusion] Characteristics of genetic variation of PCV2 isolate 201105ZJ provided theoretical basis for vaccine development, investi-gation of PCV2 pathogenesis, and prevention and control of porcine circovirus-as-sociated diseases (PCVAD) in East China.%[目的]了解我国部分地区猪圆环病毒2型（PCV2）的遗传变异特性。[方法]使用细胞传代的方法对 PCR检测为猪圆环病毒2型阳性的病料进行病毒分离，并通过 PCR、IFA进行初步鉴定，并特异性扩增出该分离病毒的全基因组，并进行同源性和系统进化树分析。[结果]该研究成功分离出1株 PCV2毒株，并命名为201105ZJ株；该分离株能在 PK15细胞上增殖，特异性 PCR检测可扩增出特异性片段，经 PCV2阳性血清作用后，呈现特异性免疫荧光，其 TCID50偏低，为102.67；基因组全长1768bp，与13株参考毒株的序列同源性介于94.1%～96.8%；与 AF055392PCV2a的同源性最高，为96.8%；分离株201105ZJ 和AF055392属于基因型 PCV2a，与 PCV2c基因型亲缘关系较远。[结论]该研究中201105ZJ分离株的遗传变异特征对于疫苗的研发、PCV2致病机理的研究和华东地区 PCVAD的防控提供了理论依据。