高效液相色谱法测定膨化食品中甜蜜素的含量

摘要

A high performance liquid chromatography method was developed for cyclamate determination in puffed food. Cyelohexanol nitrite produced by the reaction between cyclamate and nitrite under the acid environment, extracted with n-hexane, and then were determined by UV detector at the wave length of 230 rim. The method has good linearity, and the linear range is 25-500μg/mL, the detection limit is 2.5 μg/mL, the recycle rate is 96.9 %-100.4 %, RSD is 1.72 %-2.55 %. The method is simple and reliable. It can be food with satisfactory results. used to analyze cyclamate in all kinds of puffed food with satisfactory results.%建立一种高效液相色谱法测定膨化食品中甜蜜素（环己基氨基磺酸钠）含量的检测方法。甜蜜素可与亚硝酸在酸性环境下反应生成环己醇亚硝酸酯，正己烷萃取，紫外检测器于波长为230nm的条件下测定。方法线性良好，线性范围为25-500μg／mL，检出限为2．5μg／mL，加标回收率为96．9％-100．4％，RSD值为1．72％～2．55％。该方法操作方便，分析速度快，回收率高，结果准确，适用于各种膨化食品中甜蜜素含量的测定。