高效液相色谱-电喷雾电离质谱法测定地表水中微囊藻毒素

摘要

采用固相萃取-高效液相色谱-电喷雾电离质谱法测定饮用水源地地表水中痕量微囊藻毒素MC-LR,优化了色谱和质谱条件,以甲醇/水(含0.1％甲酸)混合溶液(体积比60:40)为流动相,选择定量离子质荷比(m/z)996.3.方法在0.100 mg/L-2.00 mg/L范围内线性良好,检出限为0.02 μg/L,空白水样加标回收率为(96.2±5.4)％.%Trace Microcystin ( MC -LR) in drinking source surface water was determined by HPLC -ESI - MS with solid phase extraction and test conditions of chromatography and mass was optimized. The results showed that mobile solution [ V( methanol) : V( water) =60 : 40] which contained 0. 1% methanoic acid, selective quantity ion ( m/z =996. 3). Good linearity of the method was obtained in range from 0. 100 mg/L to 2.00 mg/L, the detection limits 0. 02 μg/L, spiked standard recoveries of blank samples (96. 2 ±5.4)%.