电针对大强度运动应激大鼠细胞免疫的影响

摘要

目的:观察电针对大强度运动应激大鼠T淋巴细胞亚群、脾脏指数、胸腺指数及淋巴细胞增殖能力的影响,探讨针灸防治运动性免疫抑制的机制.方法:SD大鼠随机分为空白对照组(n=10)、模型组(n=17)、电针组(n=16).对大鼠进行为期8周、每周6次、每次150 min的游泳训练,建立运动性免疫抑制模型.电针组自第2周起每次训练后在大鼠的双侧“足三里”穴、“百会”穴与“关元”穴实施电针干预.采用流式细胞仪技术测定大鼠血浆中T淋巴细胞亚群水平；分析天平称大鼠脾脏、胸腺的重量并计算脾脏指数、胸腺指数；采用3 H-TdR渗入法测定淋巴细胞增殖能力.结果:与空白组比,模型组的CD3+水平显著降低(P＜0.05)；与模型组比,电针组的CD3+、CD4+、NK细胞(CD161+)水平明显升高(P＜0.01,P＜0.05).与空白组比,模型组的脾脏指数、胸腺指数、淋巴细胞增殖能力均显著性下降(P＜0.01,P＜0.05)；电针组脾脏指数、胸腺指数明显高于模型组(P＜0.01).结论:长期剧烈的运动应激导致大鼠的免疫功能下降,甚至抑制,但电针能明显提高CD3+、CD4+、NK细胞的水平,同时能有效防止大鼠脾脏指数、胸腺指数、淋巴细胞增殖能力的显著性下降,提示电针对运动性免疫抑制具有正向调节作用.%Objective To observe the effect of electroacupuncture (EA) on levels of T-lymphocytes, spleen index, thymus index and lymphopoiesis in strenuous exercise-induced immunosuppression rats, so as to study its mechanisms underlying improving immune function of strenuous training athletes. Methods A total of 43 SD rats were randomly allocated to control (n = 10). model(n = 17) and EA (n=16) groups. Immunosuppression model was established by forcing the rat to swim for 150 min in a water tank, once daily, 6 times a week and for 8 weeks. EA (2 Hz, <1 mA) was applied to bilateral "Zusanli" (ST 36). "Baihui" (GV 20) and "Guanyuan" (CV 4) for 20 min, once dally for 7 weeks. Spleen weight and thymus weight were detected after killing the anesthetized rats for calculating spleen Index and thymus index, separately. Peripheral blood T cell subsets were mea-sured by using flow cytometer, and lymphopoiesis was detected by using 3H-thymidine deoxyribonucleotide (3H-TdR) incorporation. Results In comparison with the control group, spleen index, thymus index, lymphopoiesis and CD3+ levels of the model group were decreased significantly (P<0. 01. P<0. 05). Compared with the model group, peripheral blood CD3+ , CD4+ and NKC levels ,and thymus and spleen indexes were increased considerably in EA group (P<0.01, P<0. 05). No significant differences were found between the control and model groups in CD4+ , CD8+ and CD4+ /CD8+ and NKC levels, and between the model and EA groups in CD8+ and CD4+ /CD8+ levels (P>0. 05). Conclusion EA intervention can effectively up-regulate peripheral blood CD3+ . CD4+ and NKC, and thymus and spleen indexes in strenuous training rats, which may contribute to its effect in relieving strenuous exercise-induced immunosuppression.