目的 研究环氧化酶-2(COX-2)、维甲酸受体β2(RAR-β2)在食管鳞状细胞癌(ESCC)及周围正常黏膜组织中差异表达状况,探讨两者间相互关系及在ESCC中的作用.方法 常规抽提33例食管癌及相应正常食管黏膜组织中总RNA,采用实时荧光定量PCR检测33例新鲜食管癌组织和正常黏膜组织中COX-2和RAR-β2基因的表达情况.结果 与正常食管黏膜组织相比,食管癌组织中COX-2的表达明显上调,而RAR-β2的表达下调(P<0.05).Ⅰ-Ⅱ级ESCC组织中RAR-β2 mRNA的表达明显高于Ⅲ级ESCC组织(P<0.05);且RAR-β2 mRNA的表达与患者是否喜好热烫饮食(T≥70℃)关系密切(P<0.05).而COX-2 mRNA的表达与患者是否喜好腌制食物显著相关(P<0.05).结论 食管鳞状细胞癌与RAR-β2、COX-2的表达有密切相关性.%To investigate the differential expression of COX-2, RAR-β2 in malignant and normal esophageal tissues, elucidate its correlation to some clinicopathologic parameters of esophageal squamous cell carcinoma and evaluate its clinic significance. Methods The expression of COX-2, RAR-β2 in 33 specimens of ESCC and corresponding noncancerous tissues were detected by real-time fluorescent quantitative polymerase chain reaction, and the relationship between COX-2, RAR-β2 and pathology of ESCC was further demonstrated. Results The expression of RAR-β2 of ESCC was significantly lower than that in normal tissues ( P < 0. 05 ), and was significantly related to the tumor differentiation between the well-moderately and the poorly differentiation tissues( P < 0. 05 ), and was also related to the hot-drinking (T≥70°C ) ( P < 0. 05 ). The expression of COX-2 in ESCC was significantly higher than that in normal tissues( P < 0. 05 ), and was related to the pickled-food ( P < 0. 05 ). They were not related with smoking excessive drinking,and the age and gender of the patients. Conclusion The ESCC is closely related to the expression of COX-2 and RAR-β2.
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