趋化因子受体CCR7在人胃癌组织中的表达及其与胃癌转移的关系

摘要

目的 观察趋化因子受体CCR7在胃癌中的表达情况,探讨CCR7表达水平与临床病理特征的关系.方法 采用Real-time PCR、Western blot及免疫组化技术检测CCR7在60例胃癌组织及相应的癌旁组织中的表达分布情况,并分析其表达差异与胃癌临床病理特征的相关性.结果 在60例胃癌组织中,CCR7在胃癌组织中mRNA表达水平均显著高于癌旁组织(P＜0.05).免疫组化检测结果显示CCR7在胃癌组织中的蛋白表达定位于细胞质,且CCR7蛋白在癌旁组织与胃癌组织中的阳性表达率分别为25％(15/60)和73％(44/60).经x2检验,两者差异有统计学意义(x2=24.000,P＜0.001).Western blot结果显示CCR7蛋白在胃癌组织中的表达水平显著高于癌旁组织.胃癌组织中CCR7表达水平在胃癌浸润深度、淋巴结转移、远处转移及TNM分期差异有统计学意义(P＜0.05),在患者性别、年龄及肿瘤大小差异无统计学意义.结论 CCR7在胃癌组织中高表达,其表达水平与胃癌侵袭转移密切相关,有望成为胃癌治疗新靶点.%Objective To investigate the expression of chemokine receptor CCR7 in gastric cancer and explore the correlation between expression and its clinicopathologic parameters of gastric cancer.Methods Real-time PCR,Western blot and immunohistochemistry were employed to detect CCR7 expression in 60 cases of gastric cancer tissues and its adjacent normal tissues.Furthermore,the correlation between the CCR7 expression levels and clinicopathological characteristics of ganstric cancer was analyzed.Results CCR7 mRNA levels was significantly increased in tumor tissues than in adjacent normal tissues in the 60 cases of gastric cancer(P ＜ 0.05).Immunohistochemistry showed that CCR7 protein was located in cytoplasmic.In addition,the positive expression rate of CCR7 in tumor-adjacent tissues and gastric cancer tissues was 25 ％ (15/60) vs 73％ (44/60),the difference was statistically significant(P ＜ 0.05).While Western blot results showed that CCR7 protein expression levels was significantly higher in gastric cancer tissues than in the adjacent normal tissues.And the CCR7 protein expression was tightly correlated with depth of infiltration,lymph nodes metastasis,distant metastasis and TNM stage (P ＜ 0.05),but not with gender,age and tumor size.Conclusion CCR7 is highly expressed in gastric cancer,and its expression level is closely related to invasion and metastasis of gastric cancer,which is expected to be a new target for gastric cancer treatment.