人肝细胞癌中miR-23a和XIAP的表达及意义

摘要

目的 探讨miR-23a和X连锁凋亡抑制蛋白(XIAP)在人肝细胞癌(HCC)中的表达、相互关系及临床病理意义.方法 建立组织微阵列,采用原位分子杂交方法分别检测150例人HCC和136例相对应的癌旁组织中miR-23a的变化;利用免疫组化两步法检测XIAP蛋白和Ki-67蛋白的表达水平.结果 HCC组织和癌旁组织中miR-23a的高表达率分别为48.7%(73/150)和77.2%(105/136),两者差异有统计学意义(P=0.001),XIAP蛋白在HCC和癌旁组织中的高表达率分别为25.3%(38/150)和22.1%(30/136),两者差异无统计学意义;miR-23a高表达率在患者中男性高于女性(P=0.037)、HBsAg阳性高于HBsAg阴性患者(P=0.022)、大肝癌高于小肝癌(P=0.021)、Ⅰ~Ⅱ期高于Ⅲ~Ⅳ期(P=0.002);而XIAP蛋白高表达在患者中小肝癌高于大肝癌(P=0.001)、Ⅰ~Ⅱ期高于Ⅲ~Ⅳ期(P=0.009).此外两者均与患者年龄、是否合并肝硬化、组织学分级无关.经Pearson相关检验,miR-23a和XIAP蛋白在HCC中的表达呈显著负相关性(rs=-0.308,P<0.01).结论 miR-23a和XIAP可能参与HCC的发生与发展.%Objective To investigate the expression and correlation of miR-23a and X-linked inhibitor of apoptosis protein(XIAP) in hepatocellular carcinoma(HCC) and their clinicopathological significance.Methods The expressions of miR-23a in 150 HCC and 136 adjacent tissues were detected by in situ hybridization.The expressions of XIAP and Ki-67 protein were detected by immunohistochemistry two-step method.Results The high expression rates of miR-23a were 48.7%(73/150) and 77.2%(105/136) in HCC tissues and adjacent non-cancerous tissues respectively,there was significant difference between the two groups(P=0.001).The high expression rates of XIAP were 25.3%(38/150) and 22.1%(30/136) in HCC tissues and adjacent non-cancerous tissues respectively, there was no significant difference between the two groups.The expression rate of miR-23a was higher in male than in female(P=0.037),HBsAg positive was higher than that of HBsAg negative patients(P=0.022), large hepatocellular carcinoma was higher than that of small hepatocellular carcinoma(P=0.021), and stageⅠ~Ⅱwas higher than that of stageⅢ~Ⅳ(P=0.002).While the high expression of XIAP protein in patients with small hepatocellular carcinoma was higher than that of large hepatocellular carcinoma(P=0.001), theⅠ~Ⅱ stage was higher than Ⅲ~Ⅳstage(P=0.009).In addition,both of them had no correlation with age, liver cirrhosis and histological grading.The expression of miR-23a and XIAP protein in hepatocellular carcinoma was negatively correlated by Pearson′s correlation(rs=-0.308,P<0.01).Conclusion miR-23a and XIAP may be involved in the development and progression of HCC.