Expression and clinical significance of miR-23a and MTSS1 in diffuse large B-cell lymphoma

摘要

The present study investigated the expression and clinical significance of micro-ribonucleic acid-23a (miR-23a) and metastasis suppressor 1 (MTSS1) in diffuse large B-cell lymphoma (DLBCL). A total of 70 cases of tumor tissues of patients with DLBCL and 30 cases of reactive lymphoid hyperplasia tissues were collected. OCI-LY10 cell was transfected with miR-23a antisense oligonucleotide (miR-23a ASO). The expression of miR-23a and MTSS1 in tumor tissues of patients with DLBCL and reactive lymphoid hyperplasia tissues were detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry. Spearmans test was used for correlation analysis was also performed for their expression. The relationship of the expressions of miR-23a and MTSS1 with the pathological parameters of patients with DLBCL was further analyzed. The DLBCL OCI-LY10 cells were cultured in vitro, and gene silencing downregulated the expression of miR-23a in OCI-LY10 cells. The expression of miR-23a was studied via RT-qPCR, and the effect of downregulation of miR-23a on MTSS1 protein expression was determined by western blot analysis. Moreover, the effects of miR-23a on the proliferation, metastasis and invasion capacities of OCI-LY10 cells were observed by both methyl thiazolyl tetrazolium (MTT) assay and Transwell chamber assay. The results of RT-qPCR showed that the mRNA expression of miR-23a in DLBCL tissues was significantly higher than that of reactive hyperplasia tissues. Immunohistochemical results revealed that the positive expression rate of MTSS1 in DLBCL tissues (30.00%) was significantly lower in comparison to reactive hyperplasia tissues (90.00%). Correlation analysis revealed that the miR-23a expression had a significant negative correlation with MTSS1 expression (r=−0.538, p=0.01). The expression of miR-23a and MTSS1 were correlated with the Ann Arbor staging, extranodal invasion and International Prognostic Index (IPI) scores of patients (p<0.05). However, they had no significant correlation with the sex and age of patients (p>0.05). After the downregulation of miR-23a expression, the MTSS1 protein expression in OCI-LY10 cells showed a significant increase. However, the proliferation, metastasis and invasion capacities of OCI-LY10 cells were obviously decreased. In conclusion, miR-23a promoted the proliferation, invasion and metastasis of DLBCL OCI-LY10 cells through the targeted inhibition of MTSS1. The high expression of miR-23a and the low expression of MTSS1 protein could be used as reference indexes for the prognosis of DLBCL.