含纳米银的抗菌水凝胶研究

摘要

为赋予水凝胶的抗菌性能,该文对含有纳米银的水凝胶进行了研究.首先在硝酸银溶液加入硼氢化钠还原剂和聚乙烯砒咯烷酮分散剂,合成纳米银；然后将不同体积的纳米银分散液加入到卡波姆溶液中,搅拌下滴加氢氧化钠溶液,制备含纳米银的水凝胶.紫外-可见光谱法的结果表明硝酸银被还原成纳米银,X-射线粉末衍射法证实纳米银被分散在水凝胶中,并通过Scherrer公式计算出纳米银的尺寸约为5 nm.溶胀性能测试结果表明水凝胶的溶胀率约为900％.抑菌性能测试结果显示,当水凝胶中纳米银浓度为10 μg/mL(低于纳米银的安全浓度25 μg/mL),水凝胶能有效抑制大肠杆菌和金黄色葡萄球菌的生长.所制备的抗菌水凝胶可望应用于创伤皮肤和皮肤疾病的外用治疗.%The silver nanoparticles-based hydrogels were investigated in order to increase the antibacterial property in this work. The silver nanoparticles were synthesized in aqueous silver nitrate solution by adding sodium borohydride as a reduction reagent and polyvinyl pyrrolidone as a dispersion aid. And then the silver nanoparticles-based hydrogels were further prepared in the carbomer solution by adding the dispersion solutions of silver nanoparticles with different volumes and sodium hydroxide solution. The result of UV-vis analysis indicated that the silver nanoparticles were successfully synthesized. The existence of silver nanoparticles in the hydrogels was confirmed by X-ray scattering analysis, and the diameters of silver nanoparticles were calculated to be about 5 nm according to the Scherrer equation. The swelling ratio of the hydrogels was determined to be 900%. The hydrogels exhibited strong antibacterial properties to Escherichia coli and Staphylococcus aureus with the content of silver nanoparticles 10 μg/mL, which is lower than the safe content of silver nanoparticles 25 μg/mL. The hydrogels are thus anticipated to be used for the treatment of wounded skin and skin disease.