沟叶结缕草八氢番茄红素基因ZmPSY的克隆、亚细胞定位及表达分析

摘要

The formation of phytoene by phytoene synthase is a key limiting step in the carotenoid biosynthesis pathway.In this study a novel gene,ZmPSY (GenBank Accession number:KY264128),was cloned from Zoysia matrella.Subcellular localization results proved that ZmPSY was localized in the chloroplasts.Expression analysis showed that ZmPSY had a higher expression level in leaves compared to roots and stems.The expression of ZmPSY in leaves also varied at different development stages,with the greatest expression seen in young leaves.qRT-PCR determination revealed that ZmPSY could be induced by exogenous abscisic acid (ABA),but suppressed by salicylic acid (SA) and methyl jasmonate (MeJA).This study paved the way for further discovery of the functional roles of ZmPSY.%八氢番茄红素合成酶(phytoene synthase,PSY)是生物类胡萝卜素合成途径中的一个关键酶.本实验从沟叶结缕草中克隆得到ZmPSY基因(Genbank登录号为:KY264128).该基因开放阅读框为1230 bp,编码409个氨基酸残基.亚细胞定位结果显示,ZmPSY定位于叶绿体中.实时荧光定量分析表明,ZmPSY基因在沟叶结缕草幼嫩的叶片中表达量最高.ZmPSY可受外施激素脱落酸(ABA)的诱导,但受外施激素水杨酸(SA)、茉莉酸甲酯(MeJA)的抑制.本实验为深入研究沟叶结缕草八氢番茄红素合成酶基因的功能奠定了基础.