扇贝多肽保护Hela细胞免受紫外线UVA氧化损伤

摘要

AIM: To study the protective effect of polypeptide isolated from Chlamysfarreri (PCF) on Hela cells damaged byultraviolet A (UVA) in vitro. METHODS: Cell proliferation was determined by MTT method; intra-cellular freecalcium [Ca2+]i and rates of apoptosis and death were measured by flow cytometry (FCM). RESULTS: PCF(0.5 %-2 %) enhanced the activities of glutathione peroxidase (GSH-px), superoxide dismutase (SOD), and catalase(CAT), and stimulated cell proliferation. The concentration of [Ca2+]i was increased while the amounts of MDA andthe rates of apoptosis and death of the cells were decreased. The differences between the PCF groups and controlgroup were significant (P＜0.05, P＜0.01). CONCLUSION: PCF protected Hela cells against damage by UVA viaits anti-oxidative mechanisms.%目的:建立紫外线UVA(辐照强度为3650μJ@cm-2)对Hela细胞氧化损伤模型.探究扇贝多肽(PCF)对Hela细胞紫外线UVA氧化损伤的保护作用.方法:MTT法测定细胞活性;酶法测定抗氧化酶(GSH-Px、CAT、SOD)活性;流式细胞仪AnnexinV法测定细胞的凋亡率和死亡率;Fluo-3AM为荧光染料,流式细胞仪测定细胞内游离Ca2+的含量.结果:PCF(0.5%-2%)能明显增加Hela细胞的增殖活性和细胞内游离Ca2+的浓度.显著提高Hela细胞GSH-Px、CAT、SOD活性,且呈量效关系.同时降低Hela细胞的凋亡率和死亡率.PCF组与模型对照组比较各项指标均有统计学意义(P＜0.05,P＜0.01).结论:扇贝多肽具有抗紫外线UVA对Hela细胞氧化损伤的作用.其机制与扇贝多肽提高抗氧化酶含量,抑制脂质过氧化有关.