扇贝多肽对紫外线B辐射人真皮成纤维细胞线粒体的保护作用

摘要

目的:研究扇贝多肽(PCF)对中波紫外线(UVB)辐射人真皮rn成纤维细胞线粒体的影响.方法:检测丙二醛(MDA)含量以rn及细胞内抗氧化酶(SOD、GSH-PX)的活性；流式细胞术测定rn线粒体膜电位；透射电镜观察细胞超微结构的变化.结果:rnUVB(1.176×10-4 J·cm-2)导致真皮成纤维细胞线粒体损rn伤，PCF(0.25％-1％)剂量依赖性地减轻UVB对线粒体的损rn伤；而且，PCF也可剂量依赖性地维持线粒体膜电位的相对rn稳定.PCF能够减少MDA的生成量，提高SOD及GSH-PX的活rn性.PCF各组与UVB模型组相比差异有显著性(P＜0.05，rnP＜0.01).结论:PCF保护成纤维细胞的线粒体免受UVB的rn损伤.%AIM: To study the effect of polypeptide from Chlamys farreri (PCF) on mitochondria of human dermal fibroblastsirradiated by ultraviolet B (UVB) in vitro. METHODS: Malondialdehyde (MDA) and antioxidant enzymes includingsuperoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) were determined by biochemical methods.Mitochondrial transmembrane potential was measured by flow cytometry. Ultrastructure of fibroblasts was ob-served with transmission electron microscope. RESULTS: UVB (1.176×10-4 J@cm-2) induced mitochondria damagein dermal fibroblast and PCF (0.25 %-1%) reduced the damage in a concentration-dependent manner. Furthermore,PCF also concentration-dependently maintained the stability of mitochondrial transmembrane potential. PCF wasable to reduce the MDA formation caused by UVB, meanwhile increased the activities of SOD and GSH-PX. Thedifferences among the PCF groups and UVB model group were significant (P＜0.05, P＜0.01). CONCLUSION:The UVB-induced mitochondria damage was alleviated by PCF in human dermal fibroblasts.