上海口岸截获的粉蝇属DNA条形码鉴定及系统发育研究∗

摘要

Species identification with dry specimen is usually difficult by traditional taxonomy method. According to the operational regulations for DNA barcode identification of medical insects at frontier ports, DNA barcodes of 31 dry specimens of Pollenia species captured at Shanghai port were generated. After comparing with the Barcode of Life Data (BOLD) system v4, all the 31 DNA barcodes are assigned into 5 species: Pollenia rudis (Fabricius, 1794), Pollenia pediculata (Macquart, 1834), Pollenia vagabunda (Meigen, 1826), Pollenia hungarica ( Rognes, 1987) and Pollenia sp.. A neighbor-joining tree was created based on the 31 barcodes of Pollenia and an inductive COⅠ sequence as an outgroup, it is revealed that these species can be differentiated by DNA barcodes. Therefore, COⅠ barcoding can overcome the shortages of traditional taxonomy, and improve the success rate in identification of flies with dry specimen.%使用传统形态学分类方法进行干制标本的物种鉴定通常比较困难.本文按照《国境口岸医学媒介昆虫DNA条形码鉴定操作规程》对上海口岸截获的粉蝇属干制标本进行DNA条形码研究.经BOLD System v4比对, 结果显示所得序列分别属于5个种: 粗野粉蝇 Pollenia rudis、伪粗野粉蝇P. pediculata、游荡粉蝇P. vagabunda、匈牙利粉蝇P. hungarica和Pollenia sp..引入1条COⅠ序列作为外群对全部序列构建系统发育树 (NJ树), 结果显示不同物种各自聚为一支.因此, COⅠ条形码技术可以弥补传统形态学分类方法的不足, 提高蝇类干制标本鉴定的成功率.