GH57家族高温淀粉普鲁兰酶的结构与功能分析

摘要

淀粉普鲁兰酶(E.C.3.2.1.1/41)同时具有淀粉酶(E.C.3.2.1.1)和普鲁兰酶(E.C.3.2.1.41)的功能,属于糖苷水解酶GH13和GH57家族.由于淀粉普鲁兰酶可以同时水解α-1,4和α-1,6糖苷键,在淀粉糖化工业中具有降低生产成本、提高生产效率和提高糖化率的作用.其中高温淀粉普鲁兰酶由于能够在淀粉工业液化条件下同时催化淀粉的液化和糖化反应,因此在淀粉糖化工业中更具有应用价值.另外,淀粉普鲁兰酶的双功能催化机制对酶学研究中也具有重要价值.该文就近年高温淀粉普鲁兰酶的结构及功能研究最新成果做以总结分析.%Amylopullulanse ( E.C.3.2.1.1/41 ) has the enzymatic activities of both α-amylase ( E.C.3.2.1.1 ) and pullulanase ( E.C.3.2.1.41) , and is classified into the glycoside hydrolase 13 and 57 families.Amylopullulanse can hydrolyze both the α-1 ,4 and α-1,6 glucosidic bonds and is valuable for decreasing cost, increasing both efficiency and dextrose equivalent in starch processing industry.Thermostable amylopullulanase is more valuable in starch saccharification industry due to its capability to catalyze both the liquefaction and saccharification processes under the industrial starch liquefaction condition.In addition,the special bifunctional catalytic mechanism of amylopullulanase is also of great value in enzymology research.The present review focuses on the structure and function of amylopullulanases and provides a brief overview on the latest studies.