In silico identification of catalytic residues and domain fold of the family GH119 sharing the catalytic machinery with the α‐amylase family GH57

摘要

Theglycosidehydrolasefamily119(GH119)containstheα‐amylasefromBacilluscirculansandfiveotherhypotheticalproteins.Untilnow,nothinghasbeenreportedonthecatalyticresiduesandcatalytic‐domainfoldofGH119.BasedonadetailedinsilicoanalysisinvolvingsequencecomparisonincombinationwithBLASTsearchesandstructuralmodelling,anunambiguousrelationshipwasrevealedbetweenthefamiliesGH119andGH57.Thisincludessharingthecatalyticresidues,i.e.Glu231andAsp373ascatalyticnucleophileandprotondonor,respectively,inthepredictedcatalytic(β/α)7‐barreldomainofGH119B.circulansα‐amylase.TheGH57andGH119familiesmaythusdefineanewCAZyclan...