Objective To investigate the application of Artemisinin in multidrug resistance of breast cancer. Methods The effects of Artemisinin combined with adriamycin on growth and proliferation of MCF-7/ADR cells were observed by using MTT assay. The expression levels of P-gp in MCF-7/ADR cells treated with Artemisinin plus adriamycin were detected by flow cytometry. Results Artemisinin of 10,20 and 40 μmol/L could induce reversal of drug resistance with the reversal index being 1.53,1.90 and 3.62 times respectively. The positive expression rate of P-gp on the surface of MCF-7/ADR cells treated with Artemisinin(0,10,20 and 40 μmol/L) was ( 33.41±4.63) ％ , ( 23.07 ± 5.48) ％, ( 21.82 ± 3.87) ％ , ( 16.62 ± 1.27) ％ respectively with the difference being significant among groups(P＜0.05) ,and the total expression level of P-gp in cells was(37.19±5.16) ％, (35.30±4.77) ％ , (37.45±5.19) ％ , ( 34.98±3.50)％ respectively with the difference being not significant among groups(P＞0.05). Conclusion Artemisinin can enhance the effect of adriamycin on MCF-7/ADR cells,indicating Artemisinin can partially induce reversal of multidrug resistance probably by influencing the exchange of P-gp between cytoplasm and cytomembrane, attenuating the expression of P-gp on cytomembrane, promoting the aggregation of adriamycin in cytoplasm and enhancing the killing effect of medicine on tumor cells.%目的 探讨青蒿素在乳腺癌多药耐药中的应用.方法 采用MTT法观察青蒿素联合阿霉素(ADR)对乳腺癌耐药株MCF-7/ADR细胞生长增殖的影响;应用流式细胞技术测定青蒿素联合ADR对MCF-7/ADR细胞P-糖蛋白(P-glycoprotein,P-gp)表达的影响.结果 10、20、40 μmol/L青蒿素实验组的细胞耐药逆转倍数分别为1.53、1.90和3.62倍.青蒿素联合ADR可抑制MCF-7/ADR细胞膜P-gp蛋白的表达,0、10、20、40 μmol/L青蒿素作用后的细胞表面P-gp表达阳性率分别为(33.41±4.63)%、(23.07±5.48)%、(21.82±3.87)%和(16.62±1.27)%,组间比较差异均有统计学意义(均 P＜0.05);各组细胞内总P-gp的表达水平分别为(37.19±5.16)%、(35.30±4.77)%、(37.45± 5.19)%和(34.98±3.50)%,各组间差异无统计学意义(P＞0.05).结论 青蒿素与ADR同共作用于MCF-7/ADR细胞,使细胞对ADR的敏感性增高,青蒿素具有部分逆转MCF-7/ADR细胞耐药的作用,其逆转作用机制可能是通过影响细胞质和细胞膜之间的P-gp交换,降低细胞膜上P-gp表达水平,提高药物在细胞内的聚集,增强药物对肿瘤细胞的杀伤作用.
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