干细胞相关基因Sox2对膀胱癌细胞增殖迁移和侵袭的影响

摘要

目的 探讨干细胞相关基因Sox2对膀胱癌细胞增殖、迁移、侵袭的影响.方法 实验分为4组:①人膀胱永生化上皮细胞株SV-HUC-1;②膀胱癌细胞株T24;③ T24细胞Sox2基因沉默组;④空白转染组,T24细胞转染空白病毒颗粒(Mock).实时定量聚合酶链式反应(qRT-PCR)和Western blot检测Sox2在各组细胞中的表达水平;MTT法检测各组细胞增殖情况;划痕实验检测各组细胞迁移能力;Transwell小室实验检测各组细胞侵袭能力;流式细胞术检测各组细胞凋亡情况.结果 T24细胞中Sox2高表达且增殖、迁移、侵袭能力都显著高于SV-HUC-1细胞,凋亡水平显著低于SV-HUC-1细胞.人为下调Sox2在T24细胞中的表达之后,细胞增殖、迁移、侵袭能力得到显著抑制,凋亡率上升.结论 膀胱癌细胞中Sox2基因呈高表达,该基因可能在膀胱癌的发展过程中起到关键作用.%Objective To investigate the effect of stem cell-associated gene Sox2 on the proliferation,migration and invasion of bladder cancer cells.Methods Four groups were established,including ①immortalized epithelial cell line SV-HUC-1 group;②bladder cancer cell line T24 group;③Sox2 gene silencing group;④blank virus particle group.The quantitative real-time poly-merase chain reaction(qRT-PCR)and Western blot were used to detect the expression of Sox2 in each group.MTT assay was used to detect the cell proliferation.Wound healing assay was performed to detect the cell migration ability,Transwell chamber assay to detect invasive ability of cells and flow cytometry to detect cell apoptosis in each group.Results The expression of Sox2 was significantly higher and the apoptotic level was significantly lower in T24 cells than in SV-HUC-1 cells.The prolifera-tive,migrating and invasive capacities of T24 cells were significantly stronger than those of SV-HUC-1 cells.Down-regulation of the expression of Sox2 in T24 cells markedly inhibited the cell proliferation,migration and invasion and increased the cell apop-totic rate.Conclusion The expression of Sox2 gene is highly expressed in bladder cancer cells,indicating a key role of Sox2 in the development of bladder cancer.