毛姜花油细胞原位拉曼光谱研究

摘要

In order to identify the essential oils ingredients in oil cells at room temperature, utmost avoid the change of essential oil active ingredients and avoid sample pretreatment and extractions which can be labor intensive.The oil cell of Hedychium villosum Wall in situ was analyzed by means of Raman spectroscopy.Fresh samples were prepared by free-hand section.Under the DXR Laser conforcal micro Raman spectrometer, the oil cells can be seen with objective lens of 10×.The sample has similar Raman spectrum with cineol.The Hedychium villosum Wall and cineol high intensity bands are present at 2 928/2 921, 647/652 cm-1,middle intensity bands are present at 540/545, 808/813, 915/920, 926/930, 1 012/1 016, 1 075/1 080, 1 270/1 273, 1 427/1 432 cm-1.Its indicates that principal component of sample is cineol.The 25 presented spectroscopic bands of Hedychium villosum Wall oil cell correlate very well with cineol.%为避免复杂的样品的制备及提取过程,最大限度避免精油活性成分变化,常温下,用拉曼光谱原位分析毛姜花油细胞中精油.样品切片后置于共聚焦显微拉曼光谱仪下,用10倍物镜可观察到油细胞.油细胞精油的拉曼光谱与1, 8-桉油精拉曼光谱非常相似.以毛姜花油细胞/1, 8-桉油精的拉曼峰为序,较强峰出现在2 928/2 921、647/652 cm-1,次强峰出现在540/545、808/813、915/920、926/930、1 012/1 016、1 075/1 080、1 270/1 273、1 427/1 432 cm-1.在油细胞中出现的强峰、次强峰与1, 8-桉油精的拉曼峰一致,说明毛姜花油细胞中油的主要成分为1, 8-桉油精.毛姜花油细胞的25条拉曼峰都与1, 8-桉油精的拉曼峰有很好的对应关系.