<i >In situIn situ        and        <i >ex situex situ        surface‐enhanced Raman spectroscopy (SERS) analysis of cell mitochondria

Yue Jing; Shen Yanting; Liang Lijia; Cong Lili; Guan Xin; Li Zhiyuan; Xu Shuping; Liang Chongyang; Shi Wei; Xu Weiqing

摘要

Abstract > As the "powerhouse" of cells, mitochondrion is the main site of cellular aerobic respiration and plays a vital role in performing many important cellular functions. The consequence of mitochondrial dysfunction could lead to a wide range of disorders and diseases. Traditional mitochondria‐related cytobiology methods always require the isolation of organelles for biological components, followed by structural analysis and quantitative analysis. In this study, we tried to assess the feasibility and reliability of the isolation‐based method and the in situ approach from the spectral fingerprint characters. Surface‐enhanced Raman scattering spectroscopy (SERS) was employed to investigate the molecular information of mitochondria from cancer cells (HepG2 and MCF‐7 cells) in situ with the assistant of mitochondrion‐targeting nanoprobes, which was compared with the isolation‐based ex situ method. The results display two methods, which show high consistency in the spectral profiles of mitochondrion, proving the feasibility of both in bioanalysis. Abundant molecular information such as protein and DNA is disclosed, and protein is one of the main components. From spectral comparison between two cancer cells, differences in protein content are observed. We believe that this comparative study will provide a valuable and recommended reference for the selection of analytical methods for different study purposes. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 作为细胞的“省电者”，线粒体是细胞有氧呼吸的主要部位，在执行许多重要的细胞功能方面发挥着至关重要的作用。线粒体功能障碍的结果可能导致各种疾病和疾病。与传统的线粒体相关的细胞生物学方法始终需要分离用于生物成分的细胞器，然后进行结构分析和定量分析。在这项研究中，我们试图评估基于隔离的方法的可行性和可靠性和原位</ i> 从光谱指纹字符中接近。使用表面增强的拉曼散射光谱（SERS）来研究来自癌细胞的线粒体的分子信息（Hepg2和MCF-7细胞）原位</ i> 随着线粒体靶向纳米素的助手，与分离为基础 ex situ </ i> 方法。结果显示两种方法，该方法显示了线粒体的光谱分布的高一致性，证明了生物分析中的可行性。公开了蛋白质和DNA的丰富分子信息，蛋白质是主要成分之一。从两个癌细胞之间的光谱比较，观察到蛋白质含量的差异。我们认为，这种比较研究将为不同学习目的选择分析方法提供有价值和推荐的参考。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35645/'>《Journal of Raman Spectroscopy: An International Journal for Original Work in All Aspects of Raman Spectroscopy, Including Higher Order Processes, and Also Brillouin- and Rayleigh Scattering》</a> <b style="margin: 0 2px;">|</b><span>2020年第4期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yue Jing&option=202" target="_blank" rel="nofollow">Yue Jing;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shen Yanting&option=202" target="_blank" rel="nofollow">Shen Yanting;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liang Lijia&option=202" target="_blank" rel="nofollow">Liang Lijia;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cong Lili&option=202" target="_blank" rel="nofollow">Cong Lili;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guan Xin&option=202" target="_blank" rel="nofollow">Guan Xin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Zhiyuan&option=202" target="_blank" rel="nofollow">Li Zhiyuan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xu Shuping&option=202" target="_blank" rel="nofollow">Xu Shuping;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liang Chongyang&option=202" target="_blank" rel="nofollow">Liang Chongyang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shi Wei&option=202" target="_blank" rel="nofollow">Shi Wei;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xu Weiqing&option=202" target="_blank" rel="nofollow">Xu Weiqing;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>State Key Laboratory of Supramolecular Structure and Materials Institute of Theoretical ChemistryJilin UniversityChangchun China;</p> <p>State Key Laboratory of Supramolecular Structure and Materials Institute of Theoretical ChemistryJilin UniversityChangchun China;</p> <p>State Key Laboratory of Supramolecular Structure and Materials Institute of Theoretical ChemistryJilin UniversityChangchun China;</p> <p>State Key Laboratory of Supramolecular Structure and Materials Institute of Theoretical ChemistryJilin UniversityChangchun China;</p> <p>Institute of Frontier Medical ScienceJilin UniversityChangchun China;</p> <p>Key Lab for Molecular Enzymology &</p> <p>Engineering of Ministry of EducationJilin UniversityChangchun China;</p> <p>State Key Laboratory of Supramolecular Structure and Materials Institute of Theoretical ChemistryJilin UniversityChangchun China;</p> <p>Institute of Frontier Medical ScienceJilin UniversityChangchun China;</p> <p>Key Lab for Molecular Enzymology &</p> <p>Engineering of Ministry of EducationJilin UniversityChangchun China;</p> <p>State Key Laboratory of Supramolecular Structure and Materials Institute of Theoretical ChemistryJilin UniversityChangchun China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4724.html" title="光谱学">光谱学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=ex situ&option=203" rel="nofollow">ex situ;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=in situ&option=203" rel="nofollow">in situ;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=mitochondria&option=203" rel="nofollow">mitochondria;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=SERS&option=203" rel="nofollow">SERS;</a> </p> <div class="translation"> 机译：前网站;现场;线粒体;SARS; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a 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<span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=靳丽&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 靳丽</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mishara R K&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Mishara R K</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kubic R&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Kubic R</a> <span> <a href="/journal-cn-8723/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 电子显微学报 </a> </span> <span> . 2008</span><span>,第006期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-11117_thesis/02022286131.html">荧光原位杂交结合细胞形态学分析食管癌脱落细胞染色体改变的研究</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=赵焕&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> 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In situIn situ and ex situex situ surface‐enhanced Raman spectroscopy (SERS) analysis of cell mitochondria

摘要