KM突变小鼠慢性炎症性皮肤病的免疫学改变

摘要

Objective To study the pathological and immunological changes of chronic dermatitis in KM mutant mice. Method The changes of external characteristics were observed by stereomicroscopy and optical microscopy, and the immune cells and inflammatory factors were observed using HE staining, immunohistochemistry and toluidine blue staining in 3-, 6-month old wild-type and KM mutant mice. Results The skin changes showed oligotrichia, scurf, rhicnosis and the histopathology showed epidermal cell necrosis, epithelial hyperkeratosis or parakeratosis, hypergranulosis, basal cell layer edema, dilatation of blood vessels in the superficial layer of dermis and infiltration of inflammation cells in the connective tissue; increase of cutaneous CD3+ and CD4+ T cells, macrophages and mast cells; and increase of inflammatory factors IL-6, IL-22, TNF-α, and lFN-γ in the 6-month old KM mutant mice and more severe in the 3-month old KM mutant mice . Conclusions The skin tissue of KM mutant mice shows chronic inflammatory changes in pathology and cell-molecular changes similar to that in human chronic inflammatory skin diseases. KM spontaneous mutant mice may become a novel animal model in research of of chronic dermatitis .%目的 观察KM突变小鼠皮肤慢性炎症的病理变化,探讨该小鼠皮肤免疫学改变.方法 通过外部特征、常规HE病理组织学、免疫组织化学、特染方法对3月龄、6月龄KM突变小鼠皮肤炎症细胞及炎症因子进行检测并与KM野生小鼠皮肤炎症细胞及炎症因子浸润进行比较.结果 KM突变小鼠皮肤毛稀、皮屑、皮皱等；组织病理表皮细胞坏死,上皮角化过度或不全,颗粒层增厚,基底细胞层水肿,真皮浅层血管扩张,结缔组织炎细胞浸润等,皮肤CD3+、CD4+T细胞、巨噬细胞、肥大细胞等增多,同时炎症因子IL-6、IL-22、TNF-α、IFN-γ等增多；且这些炎症细胞及炎症因子浸润3月龄较6月龄增多.结论 KM自发突变小鼠皮肤组织出现自发的慢性炎症病变,与人类慢性炎症性皮肤病变有相类似的病理改变和细胞分子改变,有望培育成为一种新的慢性炎症性皮肤病的动物模型.