金针菇单核体DAN3和M与其杂交双核体G1菌丝阶段基因表达差异的转录组初步分析

摘要

以金针菇(Flammulinavelutipes)单核菌株DAN3的基因组为参考,完成单核体DAN3和M及其杂交双核体G1在菌丝阶段的转录组测序与数据分析,比较两个样本间的差异基因,并对差异基因进行GO功能分析和KEGG pathway分析.结果表明:两个样本中共有显著性差异表达的基因86个,其中,在G1中呈上调、下调表达的基因数分别为41、45个,有2个基因在G1中特异表达.GO功能分析结果表明,86个差异基因中有40个基因比对上了GO功能注释,其中18个基因在G1中呈上调表达;单一生物过程和催化活性为显著性富集的功能,相关基因在G1中呈上调表达.KEGG pathway分析结果表明,22个差异基因被定位到17条Pathway,其中10个基因在G1中呈上调表达,包括赖氨酸代谢途径对应基因,3_M和G1菌丝样品中赖氨酸含量分别为1.70×103 ng/mg和1.06×103 ng/mg,说明G1中上调的基因可能与之降解相关;DNA复制是显著性富集的代谢途径,相关基因在G1中呈下调表达.%The trancriptomes of Flammulina velutipes 3_ M monokaryotic mycelium (derived from the combined culture of mycelia of monokaryons DAN3 and M) and mycelium of the hybrid dikaryon G1 (derived from crossing monokaryons DAN3 and M) were compared using the whole genome sequence of DAN3 as a reference.Both 3_M and G1 were subjected to mRNA sequencing and exposed to GO (Gene Ontology) function and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analyses.A total of 12209 genes were detected by mRNA sequence analysis from both samples of mycelium,of which 10226 were expressed and 763 were identified as believable.A total of 86 genes were differentially expressed in 3_M and G1,of which 41 were up-regulated and 45 down-regulated in G1.Two genes (11245 and 11712)were specifically expressed in G1,and two genes(10412 and 11368) expressed only in 3_M.GO analysis annotated 3270 genes and assigned a function to 40 of the 86 differentially expressed genes,of which 18 were upregulated in G1.KEGG pathway analysis revealed that 22 of the 86 differentially expressed genes were associated with 17 metabolic pathways,ten of which were up-regulated in G1,including single genes associated with lysine metabolism.Lysine levels in 3_M and G1 mycelia were 1.70 × 103 ng/mg and 1.06 × 103 ng/mg respectively,indicating that at least one up-regulated gene in G1 appears to be involved in lysine decomposition.