目的:以慢性心力衰竭大鼠为研究对象,观察燧心胶囊对左室重塑的影响,测定大鼠血清中血管紧张素Ⅱ( AngⅡ)和醛固酮(ALD)含量,以探究燧心胶囊治疗慢性心衰的作用机制.方法:将60只大鼠随机分为假手术组、模型对照组、地高辛组、卡托普利组、燧心胶囊低剂量组、燧心胶囊高剂量组.采用腹主动脉缩窄术建立大鼠慢性心力衰竭模型,连续给药12周后,处死大鼠,称取全心质量(HW)、左室质量(LVW),计算心脏重量指数(HWI)和左室质量指数(LVMI)；酶联免疫吸附法(ELISA)检测血清AngⅡ、ALD浓度.结果:与模型对照组比较,卡托普利组、燧心胶囊高剂量组大鼠HWI、LVMI显著降低,差异具有统计学意义(P＜0.01).而燧心胶囊低剂量与高剂量两组比较差异具有统计学意义(P＜0.01).血清AngⅡ、ALD检测显示:与模型对照组比较,地高辛组、卡托普利组、燧心胶囊低剂量组、燧心胶囊高剂量组大鼠血清AngⅡ、ALD浓度均显著降低,差异具有统计学意义(P＜0.05或P＜0.01).结论:燧心胶囊能够延缓或逆转左室重塑的形成,其疗效有剂量依赖关系.其机制可能是使循环中AngⅡ、ALD水平有效降低,抑制慢性心力衰竭时神经激素的过度激活,从而干预心室重塑的过程.%Objective: To research the mechanisms of Suixin capsule interfering in ventricular remodeling of rats with chronic heart failure (CHF). We measured angiotensin II (Ang II ) and aldosterone (ALD) in rats serum. Methods: Partially banding abdominal aortic artery to achieve CHF rats models. 60 rats were randomly divided into sham group, control group, digoxin group, captopril group, low - doseage group and high -dosage group and euthanized post - surgery for analysis. Heart weight index ( HWI) was determined by heart/body weight and left ventricular mass index (LV-MI) was determined by left ventricular /body weight. Ang Ⅱ and ALD level in rats serum were measured by enzyme linked immunosorbent assay (ELISA). Results; Compared with control group, HWI,LVMI in high -dosage group and captopril group were significantly increased (P <0. 01), while Ang II and ALD level in low - dosage group and high -dosage group decreased (P<0.01). Compared with control group, the density of rats serum Ang II and ALD was obviously decreased in other groups,which had a significant difference (P<0.05 or P<0.01). Conclusion; Suixin capsule can decrease Ang Ⅱ and ALD levels in CHF rat serum and inhibit ventricular remodeling with a dose - dependent manner.
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