柴胡加龙骨牡蛎汤对慢性应激抑郁大鼠海马BDNF表达的影响

摘要

目的：探讨柴胡加龙骨牡蛎汤对慢性应激抑郁大鼠海马BDNF表达的影响。方法：选择SD雄性大鼠50只，随机分为对照组、抑郁模型组（模型组）、柴胡加龙骨牡蛎汤低剂量组、柴胡加龙骨牡蛎高剂量组、氟西汀组5组，每组10只。用慢性应激结合孤养方法造模，免疫组化法观察大鼠海马BDNF的表达。结果：抑郁模型大鼠海马BDNF表达降低（ P＜0．05），柴胡加龙骨牡蛎汤可不同程度减轻上述变化。中药高剂量组与氟西汀组作用相近（P＞0．05）。结论：柴胡加龙骨牡蛎汤可增加抑郁模型大鼠海马的BDNF表达，这可能是其抗抑郁作用的环节之一。%Objective:To discuss the effects of radix bupleuri ,fossil fragments and oyster soup on the expression in chronic mild stress depression rats ’ hippocampus .Method:Randomly divide 50 SD male rats into five groups , 10 SD rats in each group, which were control group, depression model group(model group), low and high dose groups of radix bupleuri, fossil fragments and oyster soup , fluoxetine group .Chronic mild stress with solitary condition and immunohistochemistry was taken to establish model to observe the expression of the hippocampus BDNF in rats .Results:Expression of hippo-campus BDNF in depression model group was lower (P<0.05),radix bupleuri,fossil fragments and oyster soup could ease this variation to certain extent .The data in high dose group were similar to them in fluoxetine group ( P>0 .05 ) . Conclusion:Radix bupleuri ,fossil fragments and oyster soup can increase the expression of hippocampus BDNF in de -pression models .This may be one phrase in antidepression .