丹参多化学成分的UPLC-LTQ Orbitrap MS快速表征解析

摘要

目的:建立超高效液相色谱-线性离子阱/静电场轨道阱组合式高分辨质谱联用(UHPLC-LTQ Orbitrap MS)技术快速鉴别分析中药丹参中的化学成分.方法:采用电喷雾(ESI)正、负离子电离模式对中药丹参化学成分进行数据采集,根据高分辨数据及二级质谱信息对丹参化学成分进行快速鉴定.结果:归纳总结了丹参酮IIA、丹酚酸B等标准品的特征碎片及质谱裂解规律,并根据高分辨数据及二级质谱信息对丹参化学成分进行快速鉴定,在丹参提取物中共鉴定出70种化合物,包括二萜类、酚酸类、醌类等.结论:该方法可快速对丹参中的化学成分进行定性分析,能够为丹参质量控制及药效学研究提供技术支持,同时也为复杂中药成分的全面表征提供一定借鉴.%Objective:To establish a quick identification method of UPLC-LTQ Orbitrap MS ( ultra-high performance liquid chromatography-linear trap/orbitrap high resolution mass spectrometry) for the identification of chemical constit-uents in Danshen( Radix et Rhizoma Salviae Miltiorrhizae) . Methods:The negative and positive ion model was used for Orbitrap MS scanning, the components were identified by high resolution data and the secondary mass spectrum informa-tion. Results:Debris features and mass spectrum cracking rule were concluded in the standard substance of tanshinone IIA and Dan phenolic acid B. 70 components including diterpenes, phenolic acids, and quinones were discovered from extract of Radix et Rhizoma Salviae Miltiorrhizae. Conclusion: This method could provide rapid qualitative analysis for the chemical components of Radix et Rhizoma Salviae Miltiorrhizae, thus to provide technical support for the quality con-trol of it and its pharmacodynamics research. It can also provide reference for comprehensive characterization of complex chemical constituents of Chinese herbs.