以氘代尼古丁、氘代可的宁、3-OH-氘代可的宁、氘代尼古丁葡萄糖醛酸复合物、氘代可的宁葡萄糖醛酸复合物、3-OH-氘代可的宁葡萄糖醛酸复合物作内标,在LC-MS/MS的大气压化学电离(APCI)离子化模式下,建立了快速检测吸烟者尿液中尼古丁及其9种代谢物的方法,并对19个实际样品进行了测试和判别分析(DA).实验前处理简单,色谱运行时间仅为3.8 min.结果表明,尼古丁及其代谢物的精密度在0.5%～5.5%之间,回收率在94%～109%之间,线性相关系数均大于0.995.DA分析充分区别开了不同量的吸烟者,进一步的相关性分析表明吸烟者24 h尿液中的尼古丁及其9种代谢物含量与卷烟抽吸量的分析因子之间均有较强正相关性.%A fast, highly efficient method for the detection of nicotine and its nine metabolites in urine was established in this paper. The quantification was achieved by using d3-nicotine, d3-cotinine,d3-3-OH-cotinine, d3-nicotine glucuronic acid, d3-cotinine glucuronic acid and d3-3-OH-cotinine glucuronic acid as internal standards under atmosphere pressure chemical ionization (APCI) condition of LC-MS/MS.Nineteen samples were quantified and then analyzed by discriminant analysis (DA). The pretreatment of this method was simple, time-saving and all compounds were eluted within 3.8 min. The relative standard deviation (RSDi) of nicotine and its nine metabolites were between 0.5％ and 5.5％ with the recovery rate ranged from 94％ to 109％. The linear correlations were no less than 0.995. Smokers of different levels were significantly distinguished using DA and a strong positive correlation was found between the value of nicotine and its 9 metabolites in 24 h urine and smoke amount.
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