Lonicera has more species and are widely distributed in China, in which Lonicera japonica Thunb. is a traditional Chinese herbal medicinal source of honeysuckle. A high performance liquid chroma-tography (HPLC) with a photodiode array detector (HPLC-PAD) and a HPLC-electrospray ionization-multistage mass spectroscopy (HPLC-ESI-MSn) were employed for qualitative and quantitative analysis of chlorogenic acid in five Lonicera spp. plants at different flowering stages. The results indicated that buds and open flowers of all five species of Lonicera genus contained neochlorogenic acid,chlorogenic acid,chlorogenic acid methyl ester,isochlorogenic acid A and an isomers of isochlorogenic acid. Chlorogenic acid and total chlorogenic acids content were higher in flower bud than that in opening flower, but no significant variation in L. sempervirens. While the chlorogenic acids and the total content of chlorogenic acids in bud expand period (big white bud period) and the initial flowering period (silver period) of L. ×heckrottii Re-hd. ' Huoyan' ,L. sempervirens and L. × tellmanniana is higher than that of L. japonica ,but no significant differences between L. periclymenum L. ' Geleimu' and L. japonica. Consequently, flowers and buds of these four Lonicera plants had high value to exploit.%忍冬是中国传统中药材金银花的药源植物.采用高效液相色谱-光电二极管阵列检测器联用技术(HPLC-PAD)和高效液相色谱-电喷雾离子化-多级质谱联用技术(HPLC-ESI-MSn)对忍冬和4种同属植物在开花不同阶段中绿原酸类化合物进行了定性和定量分析,以明确忍冬属植物花的利用价值及其最佳采收期.结果发现:4种忍冬属植物花蕾及开放花朵中均含有新绿原酸、绿原酸、绿原酸甲酯、异绿原酸A和一种异绿原酸的异构体；绿原酸和总绿原酸在花蕾中的含量高于开放花朵,但贯月忍冬变化不显著；火焰忍冬、贯月忍冬、台尔曼忍冬中绿原酸和总绿原酸的含量在花蕾膨大期(大白期)和初花期(银花期)都高于忍冬,而格雷姆忍冬和忍冬差异不显著,研究表明,4种忍冬属植物花及花蕾都具有较高的开发利用价值.
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