桑树花青素合成相关MYB类转录因子的鉴定与功能分析

摘要

In our study,8 anthocyanins biosynthesis related MYB candidate genes were identified by using bioinformatics technologies and the expression profiles in different tissues and stages of fruits during the development and determined by transcriptome data and real time PCR.Phylogenetic analysis indicated that 4 MYB genes were closed to MYB genes from Vitis vinifera L.,Oryza sativa and Zea mays L.,while only 1 MYB gene was grouped into one cluster with those from Arabidopsisthaliana and Malusdomestica L..High level of transcripts of major genes in male flower was determined by transcriptome data.Contin-uous down-regulation expressions of MnMYBJ and MnMYB4 during the development of mulberry fruits were confirmed,while MnMYB330 had a continuous up-regulation expression.Therefore,MYB genes have positive regulation and negative regulation during the accumution of anthocyanins.%该研究通过生物信息学方法,从桑树基因组中获得了8个花青素生物合成调控关键转录因子(MYB)候选基因,利用转录组数据及实时荧光定量PCR技术,分析了各基因在不同组织及果实发育过程中的表达。聚类分析结果显示,4个MYB基因与葡萄、水稻和玉米花青素调控相关MYB基因聚为一类,仅1个MYB基因与拟南芥、苹果花青素调控相关 MYB基因聚为一类。转录组数据显示多数基因在雄花中高水平表达。实时荧光定量 PCR 结果表明,2个MYB基因(MnMYBJ 和MnMYB4)在果实发育过程中持续下调,1个MYB基因(MnMYB330)在果实发育过程中显著上调,分别与花青素在桑椹中的积累成负相关和正相关关系。因此,桑树 MYB基因家族对花青素的积累可能存在正调控与负调控两种机制。