采用沙培方法,选用不同基因型烤烟‘K326'、‘中烟90'和‘净叶黄',研究氮形态对不同烤烟幼苗叶部、根部的酶活性及可溶性蛋白含量的影响.研究发现,在NO3 -N或NH4+ -N不同处理水平下,‘净叶黄'叶部硝酸还原酶(NR)的活性较高,‘中烟90'根部NR的活性较高,在NH4+ -N的处理水平下NR的活性高于NO3- -N处理水平;‘K326'叶部和根部的谷氨酰胺合成酶(GS)的活力较高,根部的GS活力远大于叶部,并且在NH4+ -N的处理水平下叶部的GS活性低于NO3- -N处理水平,根部NH4+ -N处理水平下GS活性高于的NO3- -N处理水平;可溶性蛋白的含量具体表现为‘净叶黄'叶部较高,‘中烟90'根部较高.结果表明不同基因型烤烟间根部、叶部NR和GS活性以及可溶性蛋白含量与氮素形态有关,并且与氮素水平不呈正相关.%The effects of nitrogen forms on different flue-cured tobacco seedling roots, leaf soluble protein and associated enzyme activities were studied in different genotypes of flue-cured tobacco K326, Zhongyan 90 (ZY90) and Jingyehuang ( JYH ). The results showed that in different NO3- - N and NH4 + - N treatments, nitrate reductase (NR) activity in JYH leaves was higher than the other two genotypes, and NR activity in ZY90 roots was higher,furthermore, NR activity in NH4 + -N treatments was higher than in NO3- -N treatments. Glutamine synthetase (GS) activity in K326 leaves and roots were higher than the other two genotypes, and GS activity in root was much larger than in leaves. GS activity in leaves in NH4 + - N treatment was lower than NO3 - - N treatment level,and GS activity in roots in NH4 + - N treatment was higher than NO3 - - N treatment. Soluble protein content in JYH leaves and in ZY90 roots were the highest. There was no positive correlation between NR, GS activity and nitrogen levels.
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