植物组培过程中污染微生物种类及其季节性的变化

摘要

为研究植物组培过程中污染微生物种类及其季节性变化规律,以浙江省杭州和宁波的植物组培生产车间中的污染物为监测对象,利用NA、PDA等培养基对污染物进行分离纯化和培养.结果发现:植物组培污染物主要为细菌和真菌,其中细菌占52.13％,分布于9个种,主要为芽孢杆菌属和假单胞菌属；真菌占43.87％,分为18个种,芽枝霉(Cladosporium spp.)、黑曲霉(Aspergillus niger)、青霉(Penicillum spp.)和酵母为主要污染真菌.污染物的数量及真菌与细菌的污染比例呈现季节性变化,夏秋季是污染物的高发期,而春冬季节污染发生相对较少.%To study the varieties and seasonal characters of pollution microflora in plant tissue culture, the pollutant in Hangzhou and Ningbo plant tissue production workshops in Zhejiang province were monitored. The microbes were separated and purified through culturing on the NA and PDA media. The results showed that bacteria and fungi were the major pollutants. Bacteria accounted for 52. 13% covering 9 species, and were mainly from the genus Bacillus spp. and Pseudomonas spp. Fungi accounted for 43. 87% covering 18 species, and were mainly from Aspergillus ni-ger, Cladosporium spp. , Penicillium spp. and yeast. The total pollutants showed a seasonal change, so did fungal and bacterial contamination ratio in plant tissue production. The peak period of pollutant occurrence was in summer and autumn, while in winter and spring pollution occurred rarely.