外源ABA对低温胁迫下蝴蝶兰叶片生理指标的影响

摘要

The study was to reveal effects of exogenous ABA on chilling resistance of Phalaenopsis seedlings under low temperature stress. Leaves were sprayed with 50 mg · L ABA under room temperature for 1 day, and then plants were treated under 6℃ for 4 days. Plants treated with water were used as the control. Relative electric conductivity (REC) , malondialdehyde (MDA) and proline content, and activities of catalase (CAT) and superoxide dismutase (SOD) were determined on four days after cold treatment. The results showed that ABA treatment delayed the increase of REC and MDA content, reduced the accumulation of proline, and increased the activities of SOD and CAT. These above results indicated ABA treatment could increase the activities of antioxidant enzymes, reduce the reaction of membrane lipid peroxidation and the infiltration of membrane, and thus decrease the damage under low temperature in Phalaenopsis plants.%为研究脱落酸(ABA)对蝴蝶兰抗寒性的影响,以蝴蝶兰中苗为试材,叶面喷施50 mg· L-1脱落酸(ABA)1 d后,于6℃低温胁迫4d,以清水喷施为对照,在低温处理0,1,2,3和4d后测定叶片相对电导率、丙二醛(MDA)含量、脯氨酸(Pro)含量、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性.结果表明:喷施ABA可以延缓蝴蝶兰叶片的相对电导率和MDA含量的上升,减少Pro的累积量,提高CAT和SOD的活性.说明喷施ABA提高了蝴蝶兰叶片的抗氧化酶活性,减少了膜脂过氧化产物的积累,降低了细胞膜透性,从而减轻了低温胁迫的伤害.