桑黄总 RNA提取方法研究

摘要

为探索适合桑黄总RNA的提取方法，以桑黄菌丝体为试验材料，比较了用Trizol法、CTAB法和RNA prep pure Plant Kit试剂盒法提取后总RNA的质量。结果表明，3种方法均能从桑黄菌丝体中提取分离到总RNA，其中Trizol法能提取到纯度较高、完整性较好的总RNA，28S 及18S带型清晰无弥散，A260/A280值为1.96，RNA产率为604.64μg· g-1，提取的总 RNA适用于基因克隆与基因表达分析等分子生物学试验研究。%In order to obtain an ideal total RNA extraction method of Phellinus baumii, total RNA of Phellinus baumii was extracted with 3 methods including Trizol method , CTAB method and RNA prep pure Plant Kit method .The re-sults showed that all these methods can extract total RNA from Phellinus baumii, however , the Trizol method was the best and had high integrity and purity .The band of 28S and 18S was clear.The value of A260/A280 was 1.96 and the RNA yield was 604.64 μg· g-1 .Thus, the RNA extracted by Trizol method could be used for RT-PCR, gene cloning and gene expression and so on .