烟台黑猪SLA-DQB基因外显子2多态性及其与仔猪腹泻的关联分析

摘要

采用PCR⁃RFLP方法对烟台黑猪DQB基因外显子2的多态性进行研究，并对该位点与仔猪腹泻的关联进行了分析。结果表明，在烟台黑猪DQB基因外显子2上共检测出4种等位基因（ A、B、C、D），7种RFLP带型（ AA、BB、CC、AB、AC、BC、AD），该位点具有较丰富的多态性。卡方适合性检验显示，烟台黑猪的DQB基因外显子2没有达到Hardy⁃Weinberg平衡（ P＜0�05）。最小二乘法分析结果表明，烟台黑猪的性别和DQB外显子2的基因型均对仔猪腹泻无显著影响（P＞0�05），而性别与基因型的互作效应对仔猪腹泻具有极显著的影响（P＜0�01），在公猪群体中，AD基因型仔猪腹泻评分高于AA基因型个体（P＜0�05），在母猪群体中BB、AC、BC、CC基因型仔猪腹泻评分高于其他基因型仔猪（P＜0�05）。%Polymorphism of SLA⁃DQB exon 2 in Yantai black pigs was studied using PCR⁃RFLP, and its correlation with piglets diarrhea was analyzed. The results showed that there were four alleles ( A, B, C, D) and seven RFLP genotypes ( AA, BB, CC, AB, AC, BC, AD) , polymorphism of the DQB exon 2 was abundant. The chi⁃square test displayed DQB exon 2 of Yantai black pigs deviated Hardy⁃Weinberg equilibrium ( P0�05 ) . However, polymorphism of DQB exon 2 in different genders affected diarrhea piglets (P<0�01). In males, diarrhea score of genotype AD was higher than that of AA genotype (P<0�05), in female, scores of BB, AC, BC, CC were higher than others (P<0�05).