荷斯坦牛TLR6基因c ．640 G＞A多态性与体细胞评分的相关分析

摘要

To search the molecular markers that are associated with mastitis and to provide theoretical basis for the Holstein cattle breeding and disease resistance, the polymoprhisms of TLR6 gene in 303 Holstein cattle in Ningxia were detected by PCR-SSCP technique, and the effect of TLR6 gene c. 640G>A locus mutation on somatic cell score ( SCS) of milk and various factors was analyzed using general linear model. The results showed that there was a sig-nificant relationship between TLR6 gene c. 640G>A locus mutation and SCS and parity. The GA and AA genotype had significantly lower SCS than GG genotypes(PA locus mutation could be applied as a low SCC / SCS superior genotypes in Chinese Holstein breeding.%为寻找与乳房炎相关的分子标记，以期为荷斯坦牛的抗病育种提供理论基础，利用PCR-SSCP技术和直接测序技术对宁夏农垦303头中国荷斯坦牛的TLR6基因进行了遗传多态性分析，利用一般线性模型分析TLR6基因c.640G＞A突变位点与中国荷斯坦牛体细胞评分（ SCS）以及各个因素之间的相关性。结果表明，中国荷斯坦牛TLR6基因c.640G＞A突变位点与SCS值和胎次之间存在极显著和显著相关性，GA和AA基因型个体的SCS值极显著低于GG基因型个体（P＜0.01）。因此，在中国荷斯坦牛育种中，可尝试将c.640G＞A突变位点的GA基因型作为低SCC／SCS牛的优良基因型加以应用。