In order to clarify the physiological effects of noise on meat ducks and improve the animal welfare of meat ducks, 60 ducks were randomly divided into 3 groups. The 80 and 100 dB noise were applied by noise generator to establish stress model in experimental group 1 and 2, respectively. The control group did not apply noise stress. Liv-er, spleen, lung and kidney tissues were collected after the stress treatment. RT-PCR was used to detect the expres-sion of HSP70 and HSP90 mRNA in each tissue. The results showed that transcription levels of HSP70 and HSP90 mRNA in the liver, spleen, lung and kidney of experimental group 1 and 2 were significantly higher than those in the control group ( P<0. 01 ) , and the transcription levels of HSP70 and HSP90 mRNA in experimental group 2 were significantly (P<0. 01) higher than those in experimental group 1. Therefore, acute noise stress would increase the transcript of HSPs mRNA in the internal organs of meat ducks.%为阐明噪声对肉鸭的生理影响,提高肉鸭的动物福利,试验将60只北京鸭随机分成3组,利用噪声发生器进行80 dB(处理组1)和100 dB(处理组2)的噪声应激,对照组不进行应激.应激结束后采集肝脏、脾脏、肺和肾脏组织,采用RT-PCR技术检测各组织中HSP70、HSP90 mRNA转录水平.结果显示:处理组鸭肝脏、脾脏、肺脏和肾脏等4个组织中HSP70、HSP90 mRNA的转录水平极显著(P<0.01)高于对照组,且处理组2的HSP70、HSP90 mRNA的转录水平极显著(P<0.01)高于处理组1.表明急性噪声应激会使得肉鸭内脏组织的HSPs mRNA转录增加.
展开▼
