响应面法优化螺旋藻培养基

摘要

为了优化螺旋藻培养基的基本配方,以纯碱生产废料、硝酸钠(NaNO3)、氯化钾(KCl)、磷酸二氢钾(KH2PO4)为自变量,以螺旋藻藻蓝蛋白含量为响应值,应用Box-Behnken中心组合原理建立数学模型,进行响应面分析(RSM),确定螺旋藻培养基的最佳配方.结果表明,螺旋藻培养基最佳配方为纯碱生产废料17 g、硝酸钠(NaNO3)2 g、氯化钾(KCl)1 g、磷酸二氢钾(KH2PO4)1.6 g,在此条件下,螺旋藻藻蓝蛋白实际含量达到0.269 3 mg·L-1,与室外养殖采用的半合成培养基相比,藻蓝蛋白含量提高了23%.%To optimize the basic formula of Spirulina medium,the effects of soda ash production wastes, sodium nitrate(NaNO3), potassium chloride (KCl), potassium dihydrogen phosphate (KH2PO4) on the phycocyanin content of Spirulina were determined using the response surface methodology (RSM).Results showed that the optimal formula of Spirulina medium was soda ash production wastes 17 g, NaNO3 2 g, KCl 1 g, KH2PO4 1.6 g, which can achieve 0.269 3 mg·L-1 phycocyanin, 23% higher than that of the semi-synthetic medium used in outdoor cultivation.