大豆抗原蛋白对仔猪的免疫作用

摘要

Piglets were immunized by oral administrating or injecting soybean antigen protein immunizing agents to study the effects of soybean antigen protein immunizing agents on serum antibody titers of β-conglycinin(7S),glyci-nin(11S), and serum IgA, IgM, IgE, IgG and IL-4 levels in piglets. Seventy"Duroc×Landrace×Yorkshire"crossbred piglets(7-day-old)were randomly allocated into group A(control group), group B(7S sensitized group),group C(11S sensitized group),group D(7S orally immunized +sensitized group),group E(11S orally immunized +sensitized group),group F(7S injecting immunized +sensitized group)and group G(11S injecting immunized + sensitized group)with ten piglets each. Piglets were orally taken or injected with soybean antigen pro-tein immunizing agents according to design at 7 days old and were immunized again at 14 days old,and the piglets in group A were injected with physiological saline. All the piglets were weaned at 21 days old. From 21 days old,pig-lets in group A were fed with basal diets,and piglets in group B,D,F and group C,E,G were fed with basal diets supplemented with 5% 7S or 5% 11S,respectively for 7 d. The skin sensitization test was conducted in piglets at 27 days old. Blood samples of ten piglets from each group were taken from vena cava anterior at 7,14,21 and 27 days old, and the sera was separated to measure IgA,IgM,IgE,IgG and IL-4 levels by ELISA method and antibody titers of 7S and 11S by agar diffusion method. The results showed that the skin sensitization valuing results of piglets were group B and C>group D and E>group A,F and G,meanwhile the sensitization effect of 7S was higher than that of 11S in piglets. Serum antibody titers in group F and G were higher than those in group D and E at the age of 21 days,and serum antibody titers in group B and C were higher than those in group D, E, F and G at the age of 27 days. Serum IgG,IL-4 levels of piglets in group F were significantly(PD、E组>A、F、G组,且7S致敏性高于11S.在21日龄时,F、G组血清中抗体效价高于D、E组;在27日龄时,B、C组血清中抗体效价高于D、E、F、G组.在14日龄时,F组与A组相比,IgG、IL-4水平显著(P<0.05)升高;在21日龄时,D、E、F、G组的IgM、IgE、IgG、IL-4水平与A、B、C组相比显著(P<0.05)升高,除了G组IgG外,F、G组又显著(P<0.05)高于D、E组;在27日龄时,各处理组IgE、IgG和IL-4水平极显著(P<0.01)高于A组,且B组显著(P<0.05)高于C组,D、E组显著(P<0.05)高于F、G组.试验结果证实,7S对仔猪的致敏作用高于11S,大豆抗原蛋白免疫剂对仔猪具有一定的免疫保护作用,且对7S的免疫效果优于11S,注射免疫的效果要优于相同剂量的口服免疫.