野葛块根总RNA的不同提取方法比较研究

摘要

In this paper, the wild type and the mutant type of pueraia earthnut were used as the experimental material to compare the effects of Trizol, CTAB, SDS and modified SDS on total RNA extraction. Through analyzing the yield, purity, quality and electrophoretograms, an improved effective SDS method was preliminary developed to extract the total RNA from pueraia earthernut. The OD260/OD280 value of RNA extracted with this method varied in a range of 1. 7 ～ 1.9 and the eleetrophoretograms were integrated. This RNA extraction method is characterized by the high yield, low cost, etc. , and could be used in some experiments, such as RT - PCR, cDNA- AFLP and northern hybridization.%以块根膨大与块根不膨大突变型的野葛块根为材料,比较Trizol、CTAB、SDS及改良SDS法提取野葛块根总RNA的效果,通过RNA产量、纯度及电泳图谱分析等,初步确立了一种有效提取野葛块根总RNA的改良SDS法.该方法提取的RNA OD260/OD280值介于1.7～1.9,电泳图谱清晰完整,产量高,成本低,完全适用于RT-PCR、cDNA-AFLP及Northern杂交等分子生物学后续试验.