GM-CSF与生长抑素融合表达质粒对小鼠肌肉组织GHR和IGF-ⅠmRNA表达的影响

摘要

A total of 70 mice at 22 days age were randomized to 7 groups to detect GHR and IGF -1 mRNA expression in muscle tissues by semi - quantitative RT - PCR, and β - actin was served as the control. All mice of 7 groups were given twice immunizations at intervals of 2 weeks. Group 1 as the control was immunized with 0.85 % salt solution. Group 2-4 were given muscular injections (i m. ) with pcS/2SS ( Group 2) , pGM - CSF/SS ( Group 3) and pGM - CSF + peS/2SS ( Group 4) , respectively, while Group 5 -7 by oral administration with pcS/2SS (Group 5) , pGM - CSF/SS ( Group 6) and pGM - CSF + pcS/2SS ( Group 7) delivered by attenuated Salmonella. The results were as follows; GHR mRNA expression levels of Group 3,5 and 6 were higher than that of Group 1 and 7 (P < 0.05). IGF - I mRNA expression levels of Group 5 and 6 were higher than that of Group 1 ,2 ,4 and 7 ( P < 0. 05 ) . GHR and IGF - I mRNA expression levels of pGM - CSF/ SS were higher than that of co - immunization of pGM - CSF + pcS/2SS. The results also showed that rnGHR and IGF -1 nmRNA expression levels by the oral administration were largely higher than that of the muscular injection with the same DNA vaccine. These results suggest that GM - CSF can enhance immune effect of SS DNA vaccine and increase GHR and IGF -I mRNA expression in muscle tissue of mice. The immune effect of pGM - CSF/SS is superior to that of pGM - CSF + pcS/2SS. The immune response of oral administration is higher than that of the muscular injection.%选择70只小白鼠.随机分为7组,每组10只,雌雄各半.其中第1组为对照组,第2-4组分别肌肉注射pcS/2SS、pGM - CSF/SS和pGM - CSF+ pcS/2SS DNA疫苗；第5～7组分别口服以减毒沙门氏菌为载体的pcS/2SS、pGM - CSF/SS和pGM - CSF+ pcS/2SS DNA疫苗,以β- actin作为内参,利用相对半定量RT -PCR,检测小鼠肌肉组织GHR和IGF -Ⅰ mRNA的表达.结果表明:第3、5和6组的GHR mRNA表达显著高于1组和7组(P＜0.05),第5和6组的1GF -Ⅰ mRNA表达显著高于1组、2组、4组和7组(P＜0.05),GM -CSF与SS的融合表达质粒( pGM - CSF/SS)的GHR和IGF -Ⅰ mRNA表达高于pGM - CSF+ pcS/2SS共同免疫,同种DNA疫苗,口服免疫组的GHR和IGF -Ⅰ mRNA表达总体上比肌肉注射组要高.这些结果证明,GM - CSF可促进SS DNA疫苗的免疫效果,提高肌肉组织中GHR和IGF -Ⅰ mRNA的表达；pGM - CSF/SS效果优于pGM - CSF+pcS/2SS,口服免疫组要优于肌肉注射免疫组.