矿物元素和葡萄糖对木聚糖酶活力影响的研究

摘要

为探讨硫酸铵、氯化钙、磷酸氢二钾、植酸钙镁和葡萄糖对米曲霉产木聚糖酶能力的影响,采用5因素3水平的正交设计方法对其进行了分析.结果表明:矿物元素和葡萄糖对木聚糖酶活力均有一定的影响,其影响顺序为:硫酸铵＞磷酸氢二钾＞葡萄糖＞植酸钙镁＞氯化钙,最优组合的添加比例分别为1.00％、0.40％、0.00％、0.00％、0.00％.其最高酶活出现在固体培养的72 h,木聚糖酶活力达到537.56 U/g(P＜0.05).%To determine the effects of ammonium sulfate, calcium chloride, dipotassium hydrogen phosphate, phytin and glucose on xylanase production of Aspergillus oryzae, the orthogonal design (5 factors x 3 levels) was used in this study. The results showed that some mineral elements and glucose could affect xylanase production, the order of their influence was ammonium sulfate＞dipotassium hydrogen phosphate＞glucose＞phytin＞calcium chloride, and their optimum addition rates were 1.00% , 0.40% , 0.00% , 0.00% and 0.00% , respectively. The highest xylanase activity was 537.56 U/g at the 72nd hour after solid - state fermentation (P＜ 0.05).