顶空气相色谱法快速测定水果中仲丁胺的残留量

摘要

利用仲丁胺在水溶液中受热挥发的特性，建立了顶空气相色谱法快速测定多种水果中仲丁胺残留量的方法。用水提取样品中仲丁胺，提取液离心后，顶空进样，Rtx-17毛细管柱（30 m ×0．25 mm，0．25μm）分离，氢火焰离子化检测器（FID）测定。仲丁胺在0．01～2．00 mg／L范围内，峰面积与质量浓度呈线性关系，相关系数为0．9996，6种水果的仲丁胺平均回收率为70．22％～115．30％，相对标准偏差为1．1％～11．9％，检出限为0．01 mg／kg。该方法操作过程简单、快速、灵敏，满足仲丁胺残留分析的要求，可用于某些水果中保鲜剂仲丁胺残留量的测定。%A method has been constructed for the rapid determination of sec -butylamine residue in various fruits by means of the headspace gas chromatography based on the volatility of sec -butylamine in heated aqueous solution .Sec-butylamine in sam-ples was extracted by water , and the obtained extracting solution was centrifuged .Then it was analyzed by using headspace sampler in gas chromatography with hydrogen flame ionization detector (FID), and was separated by a Rtx -17 capillary column (30 m × 0.25 mm, 0.25 μm).There was a linear relationship between the peak area and the mass concentration of sec -butylamine from 0.01 to 2.0 mg/L with the correlation coefficient of 0.9996.The average recoveries of sec -butylamine in 6 kinds of fruits varied from 70.22%to 115.30%, the relative standard deviation was 1.1%~11.9%, and the detection limit of the method was 0.01 mg/kg.This method was simple , rapid and sensitive in application , could meet the requirements of sec -butylamine residue analy-sis, and could be used to determine the residual amount of the preservative sec -butylamine in some fruits .