黄瓜Cs-ppc基因的组织表达分析及光强对其在叶片中转录水平的影响

摘要

为了解黄瓜中ppc基因在各组织的转录水平及不同光强对叶片中该家族基因转录水平的影响,本研究对已克隆得到的Cs-ppc1、Cs-ppc2和Cs -ppc3基因的转录水平进行荧光定量PCR分析.Cs-ppc1和Cs-ppc3在多个组织中的表达量均较高,Cs-ppc2基因的组织表达差异性较大,花中的表达量高而在其他组织中的表达量均很低.对光强的响应中,Cs-ppc1的表达量及生物周期受光强的影响比其他两个Cs-ppc基因明显.Cs-ppc2基因的组织表达差异性较大,可能在花发育过程中发挥重要作用,而推测Cs-ppc1在适应不同光强调节的过程中发挥着重要作用.%To study the transcription level of ppc genes in organizations of cucumber, and influences of light intensity on them in leaves, we used fluorescence quantitative PCR to detect the transcription level three cucumber ppc genes. It showed that the transcription level of Cs-ppcl and Cs-ppc3 in many tissues were all high, while its level of Cs-ppc2 was very high in flowers and low in other tissues. By contrast, the effect of light intensity on Cs-ppcl transcription was more obvious than the other ppc genes. Cs-ppc2's high transcription level in flowers suggest its function in floral development, while Cs-ppcl may play an important role in the adaption to different light intensity.